FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_vgt2_b.3510000009879a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_vgt2_b.3510000009879a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785326
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT64740.36262284871222394TruSeq Adapter, Index 7 (97% over 35bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC31780.17800670577810437No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG26630.14916043344464822No Hit
CGCATTTTTAAATGGCGTACATGCCTCTTTTCATTCGGTAAAGAGGACTT25820.14462344692229878No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT23150.12966819505233218No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA22380.1253552572471358No Hit
CCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAG21370.11969802713902111No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT21330.11947397842186805No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA21290.119249929704715No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC19930.11163227332151103No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG18580.10407062911759533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCCC200.006952664352.524
TATCTCG11400.049.73684341
GTATCTC11650.048.66952540
GTATGCC12550.048.52589447
AGTCACG12900.046.93798428
CGTATGC13450.046.0594846
CGTATCT12450.045.5421739
CTCGTAT13550.044.42804344
GCCGTCT13650.043.58974551
ACTCCAG14150.043.03886823
ATTCGTA14300.042.8321736
GTCACGA14150.042.7915229
CACGAAT14400.041.80555331
TCGTATG14550.041.61511645
TTCGTAT13900.041.5467637
TCCAGTC14650.041.3310625
ACGAATT14750.041.28813632
TATGCCG14850.041.01009848
CTCCAGT15100.040.56291224
AATTCGT15150.040.42904335
Produced by FastQC (version 0.11.2)