FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_vgt1_n.351000000987ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_vgt1_n.351000000987ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609584
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT173990.6667346213036254TruSeq Adapter, Index 8 (97% over 36bp)
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG34310.1314768944015598No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT26340.10093562805412663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGCG25800.060.09689734
GTATGCC25250.059.88118747
CTCGCGC26000.059.09615735
TCGCGCA26900.058.0297436
CGTATGC26700.057.80898746
CATCTCG23100.057.4242441
CGCGCAT24100.057.36514337
CTCTCGC27100.057.0848733
CTCCAGT28350.056.0493824
TCCAGTC28350.055.92592225
GCATCTC24500.053.71428340
ACTCCAG29750.053.6470623
CTCGTAT28450.053.02284644
TGAACTC30050.052.99500720
TCGTATG29400.052.14285345
GAACTCC30600.051.92810421
CACGTCT31450.051.7488114
GCCGTCT29650.050.4047251
ACACGTC32300.050.38699313
TATGCCG30900.049.04530748
Produced by FastQC (version 0.11.2)