FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_vgt1_b.3510000009878d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_vgt1_b.3510000009878d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226463
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT37350.1677548650033708TruSeq Adapter, Index 13 (97% over 38bp)
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT24090.10819851935558776No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG22480.10096731901675438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC7700.037.72727247
CGTATGC8150.036.07361646
GATTAGT5800.035.603459
AATCTCG7600.033.6184241
TCGTATG9850.031.97969445
ACTCCAG9450.030.7407423
CTCCAGT10000.030.09999824
TCCAGTC9550.030.05235325
AGTCACA9600.029.89583228
CACGTCT9850.029.84771514
GAACTCC9950.029.54773921
CGATTAG7150.029.3706288
ACACGTC10350.029.08212713
CTCGTAT9950.028.14070344
ACCCTAG500.00721179828.00000223
TTAGTAT7550.027.35099211
TATGCCG11400.025.78947348
CGATCGA8600.025.6395364
TGAACTC11350.025.28634320
GACCTAT8050.025.21739253
Produced by FastQC (version 0.11.2)