FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_oat2_n.351000000987dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_oat2_n.351000000987dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385834
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT287202.072398281468055TruSeq Adapter, Index 6 (97% over 36bp)
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT35970.2595548961852574No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC16470.11884540284045562No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC16270.11740222854973972No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT16250.11725791112066812No Hit
CCGGGATGGACGCACCGCTGGTGTACCAGTTGTTCTGCCAAGGGCATCGC16240.11718575240613233No Hit
GTCAGCTCCATGTGTCGCCACACTTCCACCTCAGACCTATCAACCTGATC16230.11711359369159655No Hit
CCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAG15910.11480451482645108No Hit
GGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCCCAA15730.11350565796480676No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCAC15640.1128562295339846No Hit
CGCATTTTTAAATGGCGTACATGCCTCTTTTCATTCGGTAAAGAGGACTT15600.1125675946758414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC37350.066.3453847
CTCCGCG37850.065.9313133
CTCCAGT38650.065.2910824
CGTATGC37950.065.2042246
TCCAGTC38700.065.0258425
GCCGTCT36850.064.9660851
TCGTATG38000.064.7545
ACTCCAG39250.064.5605123
CCGCGAA38750.064.49032635
CTCGTAT37300.064.2761444
AATCTCG34100.063.94428341
GAACTCC40250.063.30434821
CGCGAAA39500.063.26582336
TGAACTC40400.063.1559420
CACGTCT40500.062.9135814
TATGCCG39500.062.91139248
ACGTCTG40800.062.53676215
TGCCGTC38950.062.45186250
CGAAATC35750.062.0699338
ACACGTC41400.061.71497713
Produced by FastQC (version 0.11.2)