FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_oat2_b.35100000098770.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_oat2_b.35100000098770.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374789
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT128150.9321430415867453TruSeq Adapter, Index 7 (97% over 38bp)
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT31670.23036262291886248No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC20210.14700437667162014No Hit
CCGGGATGGACGCACCGCTGGTGTACCAGTTGTTCTGCCAAGGGCATCGC15110.1099077749385542No Hit
GTCAGCTCCATGTGTCGCCACACTTCCACCTCAGACCTATCAACCTGATC14950.10874395998222272No Hit
GGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCCCAA14700.10692549911295479No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA14470.10525251511322828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG14800.064.56081441
AGTCACG17100.063.65497228
CTCCAGT17750.061.71830724
GTATGCC17550.061.42450347
CGTATGC17950.060.0557146
ACTCCAG18300.060.05464623
TCACGAG18150.059.77961330
TCCAGTC18300.059.6721325
CACGTCT18650.059.11527614
ACGTCTG18850.058.67374415
CTCGTAT17850.058.62745344
GTCACGA18550.058.49056229
TCGTATG18650.058.1769445
ACACGTC19150.058.12010613
GAACTCC19000.058.02631821
GAGATTC18450.057.85907734
GCCGTCT17850.057.8431451
CGAGATT18500.057.70270533
TGAACTC19350.056.9767420
TATGCCG19100.056.62303548
Produced by FastQC (version 0.11.2)