FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_oat1_b.35100000098763.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_oat1_b.35100000098763.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621087
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT65270.4026310740879422No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT65090.40152070801875533TruSeq Adapter, Index 2 (97% over 37bp)
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG45460.28042911947353844No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA39390.24298510814040208No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT34980.2157811394453228No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA34710.21411559034154246No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC30490.18808367471949378No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT29270.18055786025056025No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG29200.18012605122365424No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGAT28000.1727236107624082No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT27470.16945419955869118No Hit
TTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCC27070.16698671940494247No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC27030.16673997138956761No Hit
CTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACCGTCAAGGTACCG25870.15958427894369642No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG25770.15896740890525923No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT25530.15748692081301005No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA25490.15724017279763516No Hit
GCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAAAGCCTC25360.15643824174766685No Hit
CCGGGATGGACGCACCGCTGGTGTACCAGTTGTTCTGCCAAGGGCATCGC25290.1560064327207608No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT23680.14607482510192235No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA22920.14138661280979986No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC22560.13916588067142605No Hit
CACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCC22410.1382405756137703No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC22350.13787045359070796No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA22330.13774707958302054No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT22120.13645165250230246No Hit
GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC21650.13355236332164777No Hit
GGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGA21620.1333673023101166No Hit
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCA21520.13275043227167943No Hit
CCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACC21460.13238031024861713No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA21040.129789456087181No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG21000.12954270807180615No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT20760.12806221997955694No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA20410.12590317484502683No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA20300.12522461780274594No Hit
CCCACCGATGCCTCCACCGAAGCTGGCGCTCCGGTTTCCAAGGCTCCTAC19910.12281882465284097No Hit
CGGGGCTAAACGTATCACCGAAGCTGCGGACTGTTCTTCGAACAGTGGTA19580.12078315352599829No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG19460.12004290947987369No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC19320.11917929142606165No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT19290.11899423041453049No Hit
CGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGT19250.11874748239915561No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT19020.11732868131075014No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT18970.11702024629153154No Hit
GTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCGGGCT18720.11547807119543861No Hit
TCCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCT18710.1154163841915949No Hit
CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCAC18430.11368914808397082No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT17990.11097491991484726No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACG17880.11029636287256638No Hit
CCGACCCGCACGAAAGGCGCAACGATCTGGGCACTGTCTCAACGAGAGAC17880.11029636287256638No Hit
GGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCA17650.10887756178416087No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCAC17590.10850743976109857No Hit
GTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTG17380.10721201268038051No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA17270.10653345563809963No Hit
GTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCA17210.10616333361503731No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA16980.10474453252663181No Hit
TGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGC16850.1039426014766635No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC16790.1035724794536012No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG16790.1035724794536012No Hit
CGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGGGCTG16780.10351079244975749No Hit
CAAGAGTCCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCG16610.10246211338441429No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA16490.10172186933828968No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA16380.10104331229600878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGT150.00222506970.000011
GTATGCC7650.068.6274547
CGTATGC7850.067.3248446
TCGTATG7750.067.2903245
CTCGTAT7850.066.4331244
GCCGTCT7650.066.33987451
TATGCCG7950.066.03773548
CGTCTTC7600.064.4736853
ATGCCGT8150.063.98773249
TGCCGTC8100.063.95061550
CCGTCTT7800.063.7179552
ATTATCT7750.063.67741439
TATCTCG7850.063.312141
ACCGCTC8350.063.2934132
GTCTTCT7750.062.7741954
CTCCAGT8900.061.74157324
TCCAGTC8850.061.69491625
CTCATTA8400.061.66666436
TCATTAT8150.061.41104537
GCTTGAA7950.061.1949761
Produced by FastQC (version 0.11.2)