FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_79t2_n.351000000987b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_79t2_n.351000000987b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582762
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT120700.762590964402734TruSeq Adapter, Index 3 (97% over 36bp)
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG56010.35387506144322395No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA43260.27331967787955486No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT42010.2654220912556657No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT32370.20451590321223279No Hit
GCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAAAGCCTC31940.20179913341361494No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT31880.20142004925566823No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT31730.20047233886080154No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC31680.20015643539584602No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG30350.19175340322802797No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA30210.1908688735261524No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGAT27410.17317827948864073No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG27240.1721042077077918No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA26490.16736565573345832No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT26330.16635476464560053No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA25600.1617425740572493No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC25560.16148985128528484No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT24500.15479269782822686No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC24390.15409771020532462No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG23600.1491064354590267No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA23460.1482219057571511No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC23330.14740055674826663No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC23160.1463264849674177No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC23160.1463264849674177No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT22270.14070340329120867No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG21720.13722846517669746No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA21470.13564894785191964No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA21320.13470123745705292No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG20420.12901497508785276No Hit
CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCAC20390.12882543300887941No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA20260.12800408399999494No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT19890.12566639835932375No Hit
CACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCC19590.12377097756959038No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA19490.12313917063967925No Hit
CCGACCCGCACGAAAGGCGCAACGATCTGGGCACTGTCTCAACGAGAGAC19420.12269690578874146No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT19150.1209910270779814No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG19130.12086466569199918No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT19030.12023285876208804No Hit
CCTGATTCCACACTGCCAAGAAAAGCCTCTAGCGAGGTGAGAGGTGCCCG18890.11934832906021246No Hit
CCGGGATGGACGCACCGCTGGTGTACCAGTTGTTCTGCCAAGGGCATCGC18800.11877970282329245No Hit
GGCAAATCCGTTTCCCATAAGGCTGAGCTGTGATGGCGAGCGAAATATAG18780.11865334143731023No Hit
TGGCGAGCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAA18360.11599975233168348No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA18010.11378842807699453No Hit
GCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTC17990.1136620666910123No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA17930.11328298253306561No Hit
CCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTC17910.1131566211470834No Hit
GGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCA17870.11290389837511894No Hit
TGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTC17750.1121457300592256No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC17480.11043985134846554No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT17340.10955532164658995No Hit
CGGGGCTAAACGTATCACCGAAGCTGCGGACTGTTCTTCGAACAGTGGTA17310.1093657795676166No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT17270.10911305679565216No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT17240.10892351471667881No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT17210.10873397263770547No Hit
GGGAAACAGCCCAGACCGCCAGCTAAGGTCCCAAAGTATACGTTAAGTGG17190.10860761125172326No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATC17150.1083548884797588No Hit
CCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACC16930.10696491323395432No Hit
TGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGC16630.10506949244422092No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT16420.10374269789140755No Hit
TGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATAT16230.10254226472457642No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT16080.10159455432970971No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGC15840.10007821769792301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC15400.066.3636346
CATCTCG14350.066.09756541
TATGCCG15750.064.88888548
TCGTATG15750.064.88888545
GTATGCC15700.064.8726147
GCATCTC14600.064.72603640
ACTAATG15650.064.6325932
GCCGTCT14950.064.6153951
GCGCATC14700.064.523838
ATGCGCA15950.064.5141136
TGCGCAT15100.064.205337
CACTAAT15700.064.2038231
CTCGTAT15750.063.7777844
GTCTTCT14550.063.5051554
TGCCGTC15600.063.4935950
CCGTCTT15100.063.27814552
CGTCTTC15050.062.79069553
CTTGAAA15150.061.91419262
ATGCCGT16250.061.1692349
TCCAGTC17100.060.3801225
Produced by FastQC (version 0.11.2)