FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_79t2_b.35100000098757.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_79t2_b.35100000098757.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583852
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG127650.4940298438145838No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT106460.4120205027222921TruSeq Adapter, Index 6 (97% over 36bp)
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA73150.2831044502548907No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA64790.2507496559400461No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT63440.24552489848489772No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA58600.226793175460514No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT57490.2224972637751698No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT55160.21347971942665445No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGAT54590.21127371072336962No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT53240.20604895326822126No Hit
GCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAAAGCCTC52120.20171433967580188No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC51910.20090159962722326No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC50610.19587035170745073No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC50080.19381915063246657No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA49560.19180665146455755No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT47030.18201506897453879No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC46570.18023478124908082No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT45130.1746617066302559No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC44310.17148815025009173No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT43130.1669213252152213No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA42120.16301243260062884No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG41130.1591809438001867No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA39870.15430450350871489No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC39640.1534143596459859No Hit
CCCACCGATGCCTCCACCGAAGCTGGCGCTCCGGTTTCCAAGGCTCCTAC39400.15248551387618176No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG38080.14737686214225892No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA37850.14648671827952994No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA37810.14633191065122925No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT37050.1433905657135161No Hit
CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCAC36470.14114585510315605No Hit
GCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTC36070.13959777882014912No Hit
CACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCC35940.1390946540281719No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG35560.13762398155931532No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA35160.1360759052763084No Hit
CCTGATTCCACACTGCCAAGAAAAGCCTCTAGCGAGGTGAGAGGTGCCCG34720.1343730213650008No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC34600.1339085984800987No Hit
TGGCGAGCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAA34160.1322057145687911No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT33920.13127686879898695No Hit
GGGAAACAGCCCAGACCGCCAGCTAAGGTCCCAAAGTATACGTTAAGTGG33880.13112206117068625No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG33800.13081244591408486No Hit
CCGACCCGCACGAAAGGCGCAACGATCTGGGCACTGTCTCAACGAGAGAC33120.12818071623297309No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG33030.12783239906929655No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGC32630.12628432278628962No Hit
TGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTC31600.12229802635754679No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT31500.12191100728679506No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT30630.118543941371255No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA30630.118543941371255No Hit
GGCAAATCCGTTTCCCATAAGGCTGAGCTGTGATGGCGAGCGAAATATAG30600.1184278356500295No Hit
CCGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGA30240.11703456699532326No Hit
CCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTC29250.11320307819488112No Hit
TGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGC29080.11254514577460319No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT28820.11153889619064869No Hit
CCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACC28610.11072615614207006No Hit
GTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGG28460.11014562753594245No Hit
CCGGGATGGACGCACCGCTGGTGTACCAGTTGTTCTGCCAAGGGCATCGC28410.1099521180005666No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG27970.10824923408925897No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT27690.10716558069115414No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG27610.10685596543455275No Hit
GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC27420.10612062920012447No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATC27270.10554010059399686No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG27170.10515308152324514No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCAC27010.10453385101004238No Hit
CCTGGGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCAC26190.1013602946298782No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT26110.10105067937327679No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATT26100.10101197746620162No Hit
TGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATAT26020.10070236220960024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC12150.063.6625547
CTCGTAT12050.062.7385944
GCCGTCT11900.062.05882651
CGTATGC12700.061.4566946
TGCCGTC12650.060.8695650
TATGCCG12750.060.66666448
TCGTATG12700.060.6299245
AATCTCG11550.060.60605641
AGAATCT11750.059.5744739
ATGCCGT13100.058.7786349
CGTCTTC12400.058.7096853
TCCAGTC13300.058.42105525
GAATCTC12000.058.33333640
GTCTTCT12500.058.2454
ACTCCAG13500.058.07407423
CCGTCTT12750.057.92156652
GGAGAAT12600.057.77777537
CTCCAGT13700.056.970824
TGAACTC13850.056.60649520
GAACTCC13900.056.40287821
Produced by FastQC (version 0.11.2)