FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_79t1_n.351000000987a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_79t1_n.351000000987a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919587
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT125730.6549846399251505TruSeq Adapter, Index 19 (97% over 38bp)
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG58980.30725359152776094No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA49900.2599517500378988No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT44520.2319248880097646No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT44380.23119556446256406No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG39640.206502752935918No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC34760.18108061786207139No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT32300.16826536124697655No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT31730.16529597251908876No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA30070.15664827903085404No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG26900.14013431014067088No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA24700.1286735115418056No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG24660.1284651333854626No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT23760.12377662486774499No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA23480.12231797777334395No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC23220.12096351975711443No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA21900.11408704059779526No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT21810.1136181897460235No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC20870.10872130307196287No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC20750.10809616860293386No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA20730.10799197952476236No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA20440.10648123789127556No Hit
GCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAAAGCCTC20110.10476211810144578No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGAT19900.103668132780645No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG19640.10231367476441547No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG19590.10205320206898671No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA19490.10153225667812919No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA19460.10137597306087195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC16350.062.0795147
TCGTATG17400.059.74137545
CGTATGC17350.059.71181546
CTCGTAT17350.059.30835744
GAAGCTA17400.058.3333336
TATCTCG14900.058.0201341
TCCAGTC18950.056.33245525
ACTCCAG19000.056.1842123
TGAACTC19450.054.70437220
GCCGTCT18000.054.44444751
CTCCAGT19700.054.18781724
GAACTCC19650.053.96946321
TATGCCG18850.053.474848
CCCACTA200.00695274452.566
CCGTCTT18400.051.92934852
CCTGAAG20300.051.72413633
ATGCCGT20000.050.57499749
TGCCGTC19750.050.50632550
CGTCTTC19100.050.02617653
GTCTTCT19200.049.58333254
Produced by FastQC (version 0.11.2)