FastQCFastQC Report
Thu 27 Jul 2017
000000000-B5WD2_l01n01_79t1_b.3510000009874a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B5WD2_l01n01_79t1_b.3510000009874a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044001
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT516732.5280320312954836TruSeq Adapter, Index 27 (97% over 39bp)
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG51650.2526906787227599No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT42770.20924647297139287No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA41840.20469657304472944No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA39940.19540107857090092No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACAC34660.16956938866468266No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT34440.16849306825192356No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC33440.1636007027393822No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA30800.1506848577862731No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT29890.14623280516986048No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA28520.13953026441767885No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT28520.13953026441767885No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT28130.13762224186778774No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGACCTCGTAT27480.13444220428463588TruSeq Adapter, Index 27 (97% over 39bp)
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG27270.1334148075270022No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGAT25940.12690796139532223No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG23010.11257333044357609No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG21820.10675141548365191No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG21700.10616433162214695No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA21460.10499016389913704No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC21240.10391384348637793No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA21000.10273967576336802No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA20890.10220151555698848No Hit
GCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAAAGCCTC20880.10215259190186306No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA20640.10097842417885314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAT61400.065.8387637
GTATGCC67250.065.8364347
CGTATGC67650.065.65410646
TACTCGA67200.065.5729236
TCGTATG67650.065.4988945
TTACTCG68000.065.0588235
CTCGTAT66550.064.89857544
TCGATCT60800.064.8190839
ACATTAC69100.064.2764132
ATTACTC69000.064.2681134
TATGCCG69200.064.0317948
CATTACT69200.064.0317933
GCCGTCT67750.064.0073851
AGTCACA69850.063.68646628
CACATTA69650.063.5175931
CCGTCTT67450.063.51371452
GTCACAT70050.063.35474429
ACTCCAG71100.063.05907423
TCCAGTC70750.062.9752625
GTCTTCT67050.062.6398254
Produced by FastQC (version 0.11.2)