FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_vww_10e3_p2.352000000a1178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_vww_10e3_p2.352000000a1178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153975
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT4380.28446176327325867No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2240.1454781620392921No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1820.11820100665692482No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1660.10780970936840395No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1640.10651079720733886No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC1630.10586134112680629No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGGA100.007123073144.740831
CGGTGCA404.8476068E-9108.5556262
CGCACTT352.7800525E-7103.386315
GTAACAA1650.092.1077964
TAGTAAC1800.076.390992
ACGAGCC406.7044675E-572.3704154
ACGAGCT300.001946386872.370414
AGCACGC502.3215143E-672.370411
AACACGA1150.069.223881
GCACGCA554.088499E-665.791282
ACACGAG1400.062.0317842
CAAGCCA350.003586100662.0317844
CGAGCCT350.003586100662.0317845
ACGCACT606.8506542E-660.3086744
TAACAAG2600.058.4530225
ACAAGAG2600.058.4530227
CACGAGC1500.053.0716323
CACGCAC701.7072107E-551.6931533
GGTGCAT858.985353E-751.0849953
TTATTCA2050.049.4236959
Produced by FastQC (version 0.11.2)