FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_vww_10e3_p1.352000000a1043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_vww_10e3_p1.352000000a1043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127582
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA4490.3519305231145459No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2770.21711526704394035No Hit
GCAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATA2250.1763571663714317No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT2210.17322192785816182No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG2060.1614647834333997No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC1740.13638287532724053No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT1580.12384192127416094No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA1520.1191390635042561No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC1510.1183552538759386No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1500.11757144424762113No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC1450.11365239610603375No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT1400.10973334796444639No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC1350.10581429982285903No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA151.235781E-4144.755782
AGGATTG500.0130.28025
GTAACAA1600.0104.043224
GAGCAGA352.7755777E-7103.396991
GAAACAC501.9645086E-10101.329044
CGGAGCA301.607488E-596.503852
GCGGAGC406.1481114E-790.472371
GAGCATC353.4545294E-582.717594
TAACAAG2050.081.204465
AGAAGCG451.2390647E-680.4198765
TAGAAAC651.5697879E-977.945422
ACAAGAG2150.077.427517
GGATTGA851.8189894E-1276.6354146
GAAGCGG502.3178036E-672.3778846
AGCATCT451.199851E-464.33595
AAACACG808.11815E-963.3306585
TTTAAGA753.7788232E-757.9023139
CTTTAAG753.7788232E-757.9023138
AACACGA902.0578227E-856.2939156
GGGATAG400.00607924254.283426
Produced by FastQC (version 0.11.2)