FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_zk-hu-0163.352000000a1234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_zk-hu-0163.352000000a1234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128095
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA3270.25527928490573404No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC3020.235762520004684No Hit
GTATATAACACTTGTTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC2400.18736094305008003No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC1830.142862719075686No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT1710.133494671923182No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC1700.13271400132714No Hit
GTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTG1480.115539248214216No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC1460.11397790702213201No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC1430.11163589523400601No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC1410.11007455404192203No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA1400.10929388344588001No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC1330.103829189273586No Hit
GCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGT1290.10070650688941803No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT100.0071217567144.740334
AGTTTGA353.456411E-582.708761
GGCTTCT608.0754035E-872.370171
GAAGCGA406.700275E-572.370166
GTTTGAA451.20050245E-464.329042
TGAAGCG451.20050245E-464.329045
GCGAAAG451.20050245E-464.329049
AAGCGAA451.20050245E-464.329047
TTCCGTG702.3472603E-762.031577
CTTCCGT753.7817335E-757.8961376
CCTATAT651.0998536E-555.669363
ATTGGAC553.236913E-452.632852
CACCCCG553.236913E-452.632858
TCTTCCG858.973584E-751.0848245
TCCTATA752.5650714E-548.246782
CGGCTCG752.5650714E-548.24678145
CCCTGTA951.9313939E-645.7074742
TATATGG803.7558733E-545.2313545
GGTCAGG657.377394E-444.535498
CCTGCAC855.371849E-542.5706863
Produced by FastQC (version 0.11.2)