FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_rt-pcr_neg_ctrl.352000000a1050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_rt-pcr_neg_ctrl.352000000a1050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTATGTAGCTCTCGCAAAACGAGAAGTATTTGGATATGTTGATATCG30.2510460251046025No Hit
GTGCTTACCGGCGATCCATTCTTCTTCCATCTTTATTCGATCCTCTCTAT30.2510460251046025No Hit
AGTAGAAACAACAGCCGGAACCTCAACACCATAATCTGCACTGGCCAAAA30.2510460251046025No Hit
TTGTCAGACAAGGTCGTAATGCCAAGTCCGGCAGTATTCCCAAAAACGCA30.2510460251046025No Hit
CTTTTAATGTCTGTCCTGTTGATACAATATCCACAATTCGATCAGCAAGC30.2510460251046025No Hit
TTCCAAAACGTCTGCGTTTTTGGGAATACTGCCGGACTTGGCATTACGAC20.16736401673640167No Hit
CTGTTATCTAAAGCCAACATGCGCCCCAACCATCTGCACCGTAGTGCGAC20.16736401673640167No Hit
CTTATGTGGAGGTTGAAGACATTCTTGCTGCAGCGGCTGCAAAAAATGAA20.16736401673640167No Hit
GTTTGAAAGGTTCAATACTCCGGCAGCAGCTACAGGCTTCGGCATCAGGC20.16736401673640167No Hit
GTTGATACAATATCCACAATTCGATCAGCAAGCCCGATCAATGGTGCAAG20.16736401673640167No Hit
GATTTAGTTTCAAGCAGAGAAGGAAAAGAGGCAGCCGGAGAACTGGCCAA20.16736401673640167No Hit
CACTTGAGGCAAGGGCATCATGTTCAGCCTGGGAAAGCAAATCCGCTGCC20.16736401673640167No Hit
GACCAAGGGGGATGGTTTCCTTATCCCCCATCTCGGCCTGCACGGTTGAT20.16736401673640167No Hit
GTAGAAACAACAGCCGGAACCTCAACACCATAATCTGCACTGGCCAAAAC20.16736401673640167No Hit
CTATAACGCCTTGTAATTTACTACGCCATGAGCAGCGTACCTTACAGAGC20.16736401673640167No Hit
ATTGCGAATTTTGTTCGGCATGCTCGATACCCTGACGAATCAGTTCAATC20.16736401673640167No Hit
TATCTGTATCGCCAATTGCTGCGACTGTTAAATCAAATTGACCGTGCTTC20.16736401673640167No Hit
TTCTTCAGCAAGGCTCGGGCGATAGCCAGGCGCTGGCGTTGGCCGCCGGA20.16736401673640167No Hit
CATCCGTACTGTTCAATAGAAGGACCGGCAATTTCTTTTCTCGGAAGGTC20.16736401673640167No Hit
GGGTTGCTCGGGCCAGTCGCCTGAGGCGCGGGTTTCTCGGGAGCTGCCAC20.16736401673640167No Hit
ACTTAAAACAAATGCTGAAGACATAGGGACAAAAGGCCAAATGTGCTCAA20.16736401673640167No Hit
GATTATCACAGAAGCGAAAAAACTGGGTATTGTCATTCAGCATGTAGACA20.16736401673640167No Hit
TTCTAAAAGAGAGAGCCCTTCTGTCAAACCATTTTCCTCACTGGATTGGA20.16736401673640167No Hit
ATCGAGCATGCCGAACAAAATTCGCAATTACCTACAAAATAATACTAATT20.16736401673640167No Hit
AATTGATTCTGTTCCGTATGCAAGAGCTGCAACGGCTTGAGCTCCCCCCG20.16736401673640167No Hit
GTTCTCTTTCAAGCATGTATGCCAGTACGATTGGAGTTATCATTGTTCCT20.16736401673640167No Hit
CTACGAAGGCGAGTACCTGCTGGGTACTTCCATCGCACGTCCGCTGATCG20.16736401673640167No Hit
GGTAAGCACTCCGTGACGGAGGCGCTGCGTTCAGGCCGGACCATTAATAA20.16736401673640167No Hit
CCCCAACCATCTGCACCGTAGTGCGACAGGCTTGGTATCGGCGAAGGCAC20.16736401673640167No Hit
TTATTTTGCAGGCCCGAATCATGTGCTGCCTACGAATGGAACCGCCCGGT20.16736401673640167No Hit
GGCCTGAACGCAGCGCCTCCGTCACGGAGTGCTTACCGGCGATCCATTCT20.16736401673640167No Hit
CCTGTATTCAATTCGGAAATCCCTTCACCATCAATCGCTAAGGCAAGAGA20.16736401673640167No Hit
CTTTAAGGAAGCTTTATTTCCTGATAATCTCTCAATTCTCATTCTGTTTC20.16736401673640167No Hit
GTATGCAAGAGCTGCAACGGCTTGAGCTCCCCCCGCTTTGTAGATTTCTT20.16736401673640167No Hit
GTAGAAACACGAGCAACGGGCACGTACTCTACCGCACCTGCAGATGTTTT20.16736401673640167No Hit
GACAAAAGGGTGACGAAGCTCTTTTTTCCTATACAGAAAAATTTGATTCT20.16736401673640167No Hit
CATAAACATCCCGCTCTTCCTCAAGCATGACATCTTTACCGGCAATTCCG20.16736401673640167No Hit
CTTCGATTGCGCGGGCATGTGCTTCTAATCCTTCTAAACGAGCGAACGCT20.16736401673640167No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG20.16736401673640167No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)