FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_prvabc59b.352000000a12ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_prvabc59b.352000000a12ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158655
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA4140.26094355677413256No Hit
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC3840.24203460338470267No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC3280.2067378903911002No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC2630.16576849138066874No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC2260.14244744886703853No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC2210.1392959566354669No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT2200.13866565818915255No Hit
GTCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT2060.12984147994075196No Hit
GTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC1890.11912640635340835No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT1750.11030222810500773No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA1730.10904163121237906No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC1650.10399924364186443No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC1610.1014780498566071No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC1590.10021745296397845No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC1590.10021745296397845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAGT458.54925E-11112.5564352
TCGAGTT501.9645086E-10101.300793
GTTTGAA554.2018655E-1092.091627
CGAGTTT554.2018655E-1092.091624
GTTCGAG608.367351E-1084.417321
AGTTTGA651.5770638E-977.923686
ATAATAC651.1021846E-555.659773
TATAGGA1051.1186785E-955.129682
ATATAGG953.1655873E-853.3162041
TCTTCCG701.7092247E-551.684085
TAGCACC450.00969929448.2384729
TAATACT657.389019E-444.5278174
TCATAAT657.389019E-444.5278171
ACTTGGT1053.855468E-641.347268
TATCATG2150.040.3856962
ATATACT1105.3074527E-639.467844
GTATCAT2250.038.5907751
AATACTG750.001493977338.5907755
GTAAATA750.001493977338.5907752
TAATATA951.03691025E-438.0830042
Produced by FastQC (version 0.11.2)