FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_pa-259459.352000000a120d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_pa-259459.352000000a120d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190320
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA4200.22068095838587645No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC3440.1807482135350988No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC2670.14029003783102145No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT2410.1266288356452291No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA2280.11979823455233292No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2170.11401849516603614No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC2140.11244220260613705No Hit
ATAATATACAGATTATAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA2010.10561160151324085No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCACGCGATGGCAG1940.10193358554014292No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAAAC100.0071233264144.748038
TCATTTG400.006085113654.280514
TTCCGTG807.257295E-645.233767
TATAGGA1307.410563E-944.5378532
ACCTACT855.3800664E-542.572952
ATATAGG1401.4259058E-841.3565831
CTTCCGT1053.853569E-641.356586
TCTTCCG1053.853569E-641.356585
GGCTTCT1053.853569E-641.356581
GACCTAC907.53645E-540.2077871
TAATCTG1209.6374515E-636.1870085
ACTATAA1251.2748205E-534.739531
ACACCGA1051.8670807E-434.4638148
CAGATTA1501.1429329E-633.774549
TGTTACT1301.66751E-533.403394
TGCTACC1301.66751E-533.403398
CACCGAA1102.4537052E-432.897289
TCTGCAC900.003651880232.166233
TTCTATC900.003651880232.166233
GTTACTG1352.1586757E-532.166235
Produced by FastQC (version 0.11.2)