FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_p6-740.352000000a11ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_p6-740.352000000a11ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142475
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGGATGAGGGGGTAGAACCAGATGACGTCGATTGCTGGTGCAACAC2370.166344972802246No Hit
CTATATGTACTTAGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAA1960.1375679943849798No Hit
GTATAGAGTGATGACTCGCAGACTGCTAGGTTCAACACAAGTTGGAGTGG1910.1340586067731181No Hit
CTTAATACATTCACCAACCTGGTGGTGCAGCTCATTCGGAACATGGAGGC1720.1207229338480435No Hit
CTCTATACACTCCATCTGTGGTCTCCCCCTTTTTTACTTCCTTGGGAGCA1670.11721354623618178No Hit
ACTCTATATCAACAGTTGGCTTGTCCTGTGCCATTACGGTAACACAACCT1450.10177224074399016No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCG451.20119876E-464.326519
ATGTAGT400.00608420954.2754946
TATAAAG701.7069924E-551.6909452
TCTGAGA450.00969218848.244883
CATGTAG450.00969218848.244885
TGTAGTG450.00969218848.244887
CTATTAA604.975457E-448.244881
TTAAGGT657.381632E-444.5337374
GTATAAA855.3761552E-542.569011
ATAAAGG855.3761552E-542.569013
AAGGTGT907.5309945E-540.2040676
GAGGGAT750.001492489238.5959057
AGGTGTT951.0355665E-438.0880667
TCTTTAC800.002049108336.1836621
TTCTATC1001.400477E-436.183663
TCTATCT1001.400477E-436.183664
CTATCTG1051.8657427E-434.460635
GGATACC850.002758800334.055213
ATCGAGC1501.1410793E-633.771416145
TCATATA900.00365047432.1632542