FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_ny238_plasmid.352000000a12e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_ny238_plasmid.352000000a12e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167151
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG3960.23691153507906024No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG3090.18486278873593337No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC3050.18246974292705398No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA2940.17588886695263564No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG2660.15913754629047985No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT2300.1376001340105653No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT2190.13101925803614695No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT2070.12384012060950877No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA2030.12144707480062937No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT1960.11725924463509042No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC1810.1082853228517927No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG1780.10649053849513314No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT1770.10589227704291329No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT1740.10409749268625375No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT1720.10290096978181405No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT1720.10290096978181405No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT1710.10230270832959419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACAAC151.2366312E-4144.750823
ATATAAG702.8321665E-972.375411
TACTGAT608.086499E-872.375417
TACAACT300.001946146572.375414
GCTTTCG502.321436E-672.3754047
TTCGACT350.00358565862.0360685
TTAGACA805.9138074E-754.2815634
TCGACTG400.00608335854.2815636
TATAAGG400.00608335854.2815632
CAGAACC858.985753E-751.0885249
TTCTAAT1152.5011104E-950.3481142
CTTCTAA1255.2314135E-946.3202671
AGATGGC1351.0328222E-842.889139
TAAGCTT855.3766315E-542.573774
ATACATC1201.9869367E-742.218993
TAGATGG1401.4239049E-841.3573768
CTTAGAC1053.850233E-641.3573763
TTACAGG951.035656E-438.09232145
TCTTAGA1157.1914783E-637.7610862
GACAGAA1355.005404E-737.527997
Produced by FastQC (version 0.11.2)