FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_mr766a.352000000a1291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_mr766a.352000000a1291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176396
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA3400.19274813487834191No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG3350.18991360348307218No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA3030.17177260255334587No Hit
GAATGGTTTTGGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCA2900.16440282092564457No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATGAGCTTGGTTTCC2300.13038844418240778No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG2110.11961722488038277No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC1970.11168053697362752No Hit
TACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAA1960.11111363069457357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTCG100.007123776144.741995
CTAAAAG100.007123776144.741993
AACGCGC100.007123776144.741999
AGTTCGA100.007123776144.741996
AGTCTGA203.88817E-4108.5564966
CGAGTCT259.4403914E-486.845194
TCGAGTC259.4403914E-486.845193
TTCGAGT259.4403914E-486.845192
TTATGGG353.4598423E-582.709713
TCCGGGG353.4598423E-582.70971145
CCTTATG300.00194679672.3709951
GTTCGAG406.706907E-572.3709951
TCTTCCG753.7897007E-757.8967935
TACAGTG1251.8189894E-1257.8967937
CTTCCGT805.918191E-754.2782486
CTGAAGC400.00608538154.2782489
TCCGTGC805.918191E-754.2782488
TATACAG2750.050.0017785
GAGTCTG450.00969404148.247335
GGCTTCT901.3335957E-648.247331
Produced by FastQC (version 0.11.2)