FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_mex-i-44.352000000a125e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_mex-i-44.352000000a125e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207031
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA5550.26807579541228127No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4680.22605310315846422No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC3650.17630209968555433No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC3160.15263414657708266No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT3110.15021904932111615No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC3020.14587187426037646No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC2890.1395926213948636No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2840.13717752413889706No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC2760.1333133685293507No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC2760.1333133685293507No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA2700.13041525182219088No Hit
ATATCAGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTA2550.1231699600542914No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG2360.1139925904816187No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC2240.1081963570672991No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC2120.1024001236529795No Hit
ATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAG2100.1014340847505929No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATT151.2369175E-4144.754844
GTCTAAT151.2369175E-4144.754841
CTAATAT300.001946590572.377423
GGCTTCT554.091131E-665.7976461
CTTCCGT651.1015874E-555.6749346
TCTTCCG651.1015874E-555.6749345
TTCCGTG858.994557E-751.089947
GCTAGCA450.00969300848.251612
GATAGAA901.3339159E-648.251611
GGTGTCG803.7617414E-545.2358865
TCGGTCA803.7617414E-545.2358869
GTATATT1652.910383E-1143.86511
TATATTA1704.0017767E-1142.574952
TATTATG1704.0017767E-1142.574954
TGTCGGT855.380218E-542.574957
ATATTAT1704.0017767E-1142.574953
AATATAC1502.6213456E-838.6012883
GTATCAA951.0363429E-438.0933761
CTGGTGA2700.037.52903145
TAATATA1553.4993718E-837.3560872
Produced by FastQC (version 0.11.2)