FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_mex-2-81.352000000a1241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_mex-2-81.352000000a1241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180781
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA5400.2987039567211156No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4220.23343161062279777No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC3540.1958170382949536No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC3130.17313766380316514No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC3070.16981873095070832No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT2480.1371825579015494No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG2400.1327573140982736No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC2400.1327573140982736No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG2310.12777891481958834No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA2240.12390682649172202No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC2170.12003473816385572No Hit
GCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGT2170.12003473816385572No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC2120.11726896078680836No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA2100.1161626498359894No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2060.1139500279343515No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC2030.1122905615081231No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAA1940.10731216222943783No Hit
ATATCAGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTA1930.10675900675402834No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC1810.10012114104911468No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGA100.0071215737144.757958
GGCTTCT1258.913048E-1152.112865
CAGATAG701.7072096E-551.6992729
TCTTCCG1301.3278623E-1050.1085249
ACAGGTT752.5674588E-548.252657
ACCTACA752.5674588E-548.252652
AGGTTTT752.5674588E-548.252659
GTATGAG604.975083E-448.252652
AACCTAC752.5674588E-548.252651
ACACAGA803.759356E-545.236866
CCTACAC803.759356E-545.236863
TCCTATA1505.4023985E-1043.4273832
TATATGG1557.4578566E-1042.0265055
CCTATAT1557.4578566E-1042.0265053
GTATCAA700.001063044841.3594171
CCATATA1105.3008553E-639.479442
AACAGGT750.001492371738.602126
TATATAA1157.192297E-637.7629472
GTATATA1157.192297E-637.7629471
TAATATA1209.630245E-636.1894872
Produced by FastQC (version 0.11.2)