FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_ibh-30656.352000000a1227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_ibh-30656.352000000a1227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229417
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTCGTTA3190.13904810890213018No Hit
ATTCATTAGAGTGTGACCCAGCCGTCATAGGAACAGCTGTCAAGGGAAAG3160.13774044643596595No Hit
GTGTAAATAAGGGAAGCCCCTGCCAAATAACTTCCTCTGAAGATATTGCA2320.1011258973833674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGAT100.0071242414144.748263
ATTTTAG100.0071242414144.748261
ATATAAC100.0071242414144.748261
TCGCTTT100.0071242414144.748268
CGTCAGA100.0071242414144.748262
GTTCGAG501.9645086E-10101.323781
TTCGAGT501.9645086E-10101.323782
CGAGTTT554.2018655E-1092.1125264
TCGAGTT554.2018655E-1092.1125263
TATAACT259.442418E-486.848952
TATTTAC1450.059.895831
GCTTTTT502.0242769E-457.89932
ATTTACA1650.052.6357272
TTTACAC1800.048.2494163
TCCGTGC657.387682E-444.5379268
TTTTCCG657.387682E-444.5379265
GGCTTTT700.001063994741.3566441
GTATTAC1451.9463187E-839.9305531
TGACCAT1451.9463187E-839.9305539
ACATCGC1105.3095537E-639.4767955
Produced by FastQC (version 0.11.2)