FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_hrv16.352000000a12c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_hrv16.352000000a12c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76345
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTG203.8785246E-4108.5642245
CCCTATG259.4170583E-486.851382
CTATATA353.446026E-582.71562
GCTAACA300.001942000472.376144145
GCTATAT651.3972567E-766.8087541
CCGGGTA350.00357804762.0366979
TCCAAGT350.00357804762.036697145
CTATATT400.00607049254.2821122
TAGGTCG400.00607049254.2821125
TCCCTAT400.00607049254.2821121
TATGTAA450.009670407548.2507635
ACACTGA750.001487249438.600616
TCAGATC1001.3933855E-436.1880728
CAGATCC1051.856315E-434.4648369
TTCAGAT1153.1666024E-431.467897
AAATCCC1000.006090495728.9504594
TATCCAG1000.006090495728.9504595
ATACCGC1600.00217845722.6175486
TAATGCT650.00806983613.3617540-44
CCCGTTC900.005215740311.258512540-44
Produced by FastQC (version 0.11.2)