FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_hrv14.352000000a12ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_hrv14.352000000a12ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224884
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATA203.8894516E-4108.557372
TTGGGCT400.006087355354.2786874
GTAGGAT752.5710298E-548.247723
TGTAGGA1102.4556016E-432.896172
TAGGATT1352.160908E-532.1651464
TAGTATA950.00476069230.4722442
TCTCACA1450.001236197324.9557172
TAAACCG1550.00182285623.345674
CATATAC2552.7333826E-622.704813
ACTAAAG2552.7333826E-622.704812
ACTCAAC1600.0021924222.616123
CTCACAG1700.003117322221.2857591
ACAGACT1700.003117322221.2857594
GTCTAGA2053.6982683E-421.1819271
GTGTCTA1750.003687728420.6775959
CTCTAAT1750.003687728420.6775951
TAATGCA1750.003687728420.6775954
GGTTGTA2155.09999E-420.1967221
CCTTTAT2205.954545E-419.7377031
AGCTTCA1900.005934472719.0451537
Produced by FastQC (version 0.11.2)