FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_hb-2016-1840.352000000a126a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_hb-2016-1840.352000000a126a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179868
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA4960.2757577779260347No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC3860.2146018191117931No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC3850.21404585584984545No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT2950.16400916227455692No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2440.1356550359152267No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC2430.13509907265327908No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG2310.12842751350990728No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA2250.12509173393822134No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC2200.1223119176284831No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC2130.11842017479484956No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC2060.114528431961216No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC1980.11008072586563479No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA702.3519169E-762.034431
GAATCGC400.006084742554.28013145
AACAGGT805.917427E-754.280136
GGCTTCT858.9912464E-751.087181
ACAGGTT901.3334266E-648.249017
TCTTCCG951.9351792E-645.709585
AGGTTTT951.9351792E-645.709589
CTTCCGT1002.7544847E-643.4241076
TAGTGGC1002.7544847E-643.4241077
TTCCGTG1053.8525686E-641.356297
CCTAATA1053.8525686E-641.356292
CTAATAG1053.8525686E-641.356293
TAATAGT1105.303462E-639.4764564
TATAGGA1355.0092603E-737.5270042
TCTATAT1251.2744935E-534.739282
CAAGGTC1051.8667504E-434.463573145
CACCGAA850.00275956334.058129
ATATGTA3000.033.7743033
ATATAGG1501.1425327E-633.7743031
TATGTAC3000.033.7743034
Produced by FastQC (version 0.11.2)