FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_fss-13025.352000000a121a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_fss-13025.352000000a121a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184206
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAA4230.2296342138692551No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4170.22637699097749261No Hit
GTATATAACACTTATTCATCCCCATTGTGGTTGGAAAAGATATGGCCTCC3300.17914725904693657No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG2970.16123253314224292No Hit
ATATAGGACATGGGCTTACCATGGAAGCTACGAGGCCCCCACACAAGGGT2670.1449464186834305No Hit
CCCCTGTACCGCATCTCGTTTCCTTCTTTGAGAAGTCCACCGAGCACCCC2660.14440354820147008No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2510.13626049097206389No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCTTGGAGGGACAGGTAC2490.13517475000814305No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATGGGCTTGGTTTCC2290.12431734036893477No Hit
ATATGTACTTGGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAACC2220.1205172469952119No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA2100.1140028012116869No Hit
ATGCTGGATGAGGGGGTAGAACCAGATGACGTCGATTGTTGGTGCAACAC2010.10911696687404318No Hit
GTCATAGGAGTCGGGGAAAAGAAGATCACCCACCACTGGCACAGGAGTGG1950.10585974398228072No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGG1860.10097390964463697No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGA100.007126328144.726382
AGATATT100.007126328144.72638145
CAGTTCG100.007126328144.726381
ACAGGTC100.007126328144.726381
TCGAGTT259.4451115E-486.835833
CTTCCGT608.367351E-1084.423726
GGCTTCT651.5788828E-977.929591
AGTTTGA300.001947767272.363196
CGAGTTT300.001947767272.363194
TCTTCCG702.8376235E-972.363195
GTTCGAG300.001947767272.363191
TTCGAGT300.001947767272.363192
TTCCGTG754.8985385E-967.538987
GTTTGAA350.003588638762.0255937
TAATATA1101.00482794E-746.04932
ATAATAT1101.00482794E-746.04931
AATATAC1151.4251964E-744.047163
CAGATTA1303.7313657E-738.9647949
CGACCGT750.001494046238.5937049
ACGACCG750.001494046238.5937048
Produced by FastQC (version 0.11.2)