FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_extraction_neg_ctrl.352000000a116b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_extraction_neg_ctrl.352000000a116b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4362
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGCGCAGGCGTCGCTGCACCTGCCGCTGTGTTCGC120.2751031636863824No Hit
GTAGAAACACGAGCACGAACACGAGCACGAACACGAATGCGAATGCGAAT110.2521779000458505No Hit
ATCTGCAACAGATCAAAGAAACAAAAGTGAAAACATGGAAGCCAGGGATG100.22925263640531865No Hit
TGTTAACTCTGTCTGGTTTCCTGGTTTCATTGGGGACTTGGTGATTCACT80.18340210912425492No Hit
ACCGTAAACCTTGCAAACATAAGGATTATCAAGACAATTTCTTCTGACTC80.18340210912425492No Hit
ACATAGAAAGTGAAACTGTGGGCCGGGCACGGTAGCTCATGCCTGTAATC70.16047684548372307No Hit
ATTCAGGGCAGTCCTCTGTGTCAATGCTGGATGCAAATGCACAGTACGAA70.16047684548372307No Hit
GTATGATAACATAAGTTTCACAAAACTTGAGACATGTCATCTTGGTTATA70.16047684548372307No Hit
CTCTAGTGGAGAATTTTAATTCAGGGCTAGTGCAGAATCATTTTTTTCTG70.16047684548372307No Hit
GCATAAGACAATCAATTTTGGGGGGAAAAAATCCCCAGTGTTCCCATAGT70.16047684548372307No Hit
GGCTCCCCATTGCTCCTTAATTTTGGTGGCGAGAAACAGAAAGGCAACAA60.1375515818431912No Hit
GAGTGAGGTGCTGAACTCCACGATCTCATTGGTCCTTAACCCTAACTTGC60.1375515818431912No Hit
GTAGAAACAACAGCCAACCCTCTTGAGACATATTCTCAAGGTATAGGTAT60.1375515818431912No Hit
GAGCTGAGATTGTGCCACTGCACTCCAACCTGGTGACAGAGCGAGACTCT60.1375515818431912No Hit
GGATATGAGAGTCCTGGACGACAGAATCCCGTGGAGTCTTGATCTCTCAT60.1375515818431912No Hit
TTACAGAGCAAGTCAAAACCAACTGGGACCCAACAAGAAAAAAACAGAAT60.1375515818431912No Hit
GTCATGTAGTATGATAACATAAGTTTCACAAAACTTGAGACATGTCATCT60.1375515818431912No Hit
CAATAGGACAGTGAATTGCAGTTATATCAGCATTACTTGACCAGTTCTAT60.1375515818431912No Hit
GTCTTATGCAGGAATTTGGAGGTGACAGTAATAGCAGGATTACCGTAAAC60.1375515818431912No Hit
CTGTAGGGTGGAGTCTGAATTCCAAGAATTGAGTTCCCCATCTGTTAAGC60.1375515818431912No Hit
ATTAAGAGGCAGCTAAAATAACAGGCAAAGATAATGAAAGTCTTAAAGAG50.11462631820265932No Hit
GAAAAAAACAGAATCATGTCCAACTGCCATTGCCATTATTCCACATGAGA50.11462631820265932No Hit
TGGTGAAACCCTGTGTCTACTAAAAATACAAAAATTAGCTCGGCATAGTG50.11462631820265932No Hit
TGCTAGTGGTGTCATATAGGATATGAGAGTCCTGGACGACAGAATCCCGT50.11462631820265932No Hit
ACATCAATATATTCTCAAAAGAAATTAAGAGCTCAGAAACTTGAGTGCTA50.11462631820265932No Hit
CCCCAGGACTCTGCTAGAAATATAATTCCCCACTTTGTGTGTTCAAAGAC50.11462631820265932No Hit
ATCCTTATGTTTGCAAGGTTTACGGTAATCCTGCTATTACTGTCACCTCC50.11462631820265932No Hit
CTCCATACTCCATCCATCCCTGGCTTCCATGTTTTCACTTTTGTTTCTTT50.11462631820265932No Hit
CTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAGAGACACTA50.11462631820265932No Hit
GTATGTAACTGGATGATGTCCTCTGGTCACAGTTTTACTTTCTTTTTTTT50.11462631820265932No Hit
TCTTTGATCTGTTGCAGATGGTAGTGTTTGTTAACTCTGTCTGGTTTCCT50.11462631820265932No Hit
GTTAACAAACACTACCATCTGCAACAGATCAAAGAAACAAAAGTGAAAAC50.11462631820265932No Hit
GTCATATAGGATATGAGAGTCCTGGACGACAGAATCCCGTGGAGTCTTGA50.11462631820265932No Hit
ACCCTACAGACACCAAATTAGTCACAGGTAGGAATATTATAGATGTTAAC50.11462631820265932No Hit
CTTTAAGACTTTCATTATCTTTGCCTGTTATTTTAGCTGCCTCTTAATTG50.11462631820265932No Hit
CATTGGGGACTTGGTGATTCACTATTTTGCGGGAAGAGGGAGGAGATGGG50.11462631820265932No Hit
GGGAGGAGATGGGGAAAGGAGAAGGTGGAGAAAAGAAGGACGTGACATTT50.11462631820265932No Hit
AGTTAGGGTTAAGGACCAATGAGATCGTGGAGTTCAGCACCTCACTCTCT50.11462631820265932No Hit
GGTCAGGAGATCGAGACCAGCCTGACCAACATGGTGAAACCCTGTGTCTA50.11462631820265932No Hit
ATTATGGGCTCTCATGTGGAATAATGGCAATGGCAGTTGGACATGATTCT50.11462631820265932No Hit
CCTTTCTACTATTGCATTTTTAAATTCCAAAATCTCTCTTTTGTCCCAGA50.11462631820265932No Hit
ATTTCATACTTAACATGAAGTATCTCAACGCTTTACAGAGCAAGTCAAAA50.11462631820265932No Hit
ACACAGAGGACTGCCCTGAATTAAGATTCAAATTACTTCTTGGCTTAACA50.11462631820265932No Hit
GTAGAAACACGAGCGGGGTCAAATCCTTTGCAGACGACTTAGCTGTGCTC50.11462631820265932No Hit
TTATTTCCCCATGCAGTGGTTCTCTGCTCCTCTCCATACTCCATCCATCC50.11462631820265932No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG50.11462631820265932No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT350.003580943420.679804125-129
Produced by FastQC (version 0.11.2)