FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_dakar-41671.352000000a1284.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_dakar-41671.352000000a1284.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154192
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGTTTTGGCGATACTAGCCTTCTTGAGATTCACAGCAATCAAACCA3260.21142471723565423No Hit
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTTGCCA2040.1323025837916364No Hit
ATTCCACACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTTCCAT1940.1258171630175366No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGT100.0071213646144.75251
TATCTGC100.0071213646144.7525145
TCCAGGG259.4353966E-486.85151145
GTTCGAG259.4353966E-486.851511
CGAGTTT300.00194576872.376264
TTCGAGT300.00194576872.376262
GGCTTTT808.134521E-963.3292247
GCTTTTT851.31331035E-859.6039778
GTATTAC753.7846257E-757.901011
ATTACTT953.1584932E-853.3298723
TTACTTG953.1584932E-853.3298724
CATTACT1056.9483576E-848.250842
TATTACT901.3318258E-648.2508352
ATTACTC951.9328581E-645.711323
TGCCTGG1151.42114E-744.0551152
CCTGTAT1002.7511906E-643.4257553
TTTTCCG855.3743046E-542.574275
TTACTCC1053.8479666E-641.357864
AATGGTT1807.2759576E-1140.209032
TCCGTGC902.2122033E-540.209038
Produced by FastQC (version 0.11.2)