FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_dakar-41667.352000000a1277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_dakar-41667.352000000a1277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174384
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGTTTTGGCGATACTAGCCTTCTTGAGATTCACAGCAATCAAGCCA3880.22249747683273693No Hit
ATTCCATACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTTCCAT3040.1743279199926599No Hit
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTTGCTA2380.13648041104688502No Hit
CTCTATTCCATACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTT2290.13131938709973393No Hit
GGCTTTTTCCGTGCCATGGCGTTATCGGCCTGACTATGAGCTTGGTTTCC2250.1290255986787779No Hit
CATCAATAGATGGGGTTCCGTGGGGAAGAAGGAGGCTATGGAAATAATAA2000.11468942104780255No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA1940.11124873841636847No Hit
GAGTTATGAGTGCCCCATGCTAGACGAGGGAGTGGAGCCAGATGACGTCG1880.1078080557849344No Hit
GTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAA1880.1078080557849344No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAGGAGCCAGGCAATAGCTA1860.10666116157445638No Hit
CTATAAGGGATCCACACCATAAGTCCTCCCTTTTCCCCAAATAGGGAATG1780.10207358473254427No Hit
TCCGTACACTACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACGTCAT1750.10035324341682725No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTC100.007124437144.737036
CGAGTTT301.6101882E-596.491354
GTTCGAG301.6101882E-596.491351
TTCGAGT301.6101882E-596.491352
GCTTTTT451.2018534E-464.327572
TCCGTGC502.0236992E-457.894818
CAAGTAG553.2405357E-452.631643
TGAAGCG450.00969517248.2456745
AAGCGAG450.00969517248.2456747
GGCTTTT604.978142E-448.2456741
ACATTGT803.7620142E-545.230326
TTACATT855.3806154E-542.569714
AAGTAGC700.001063697541.3534364
GTAGCAT700.001063697541.3534366
AGCATCT750.001493287338.596548
TAGTCCG750.001493287338.596549
GCATCTG750.001493287338.596549
TACATTG951.0364209E-438.088695
AAATAGT800.002050200536.1842586
GAAATAG850.002760266734.055775
Produced by FastQC (version 0.11.2)