FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_472.352000000a115f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_472.352000000a115f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65883
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT6130.9304372903480412No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2680.4067817191081159No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2090.31722902721491125No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1770.26865807567961386No Hit
GTATGCAGAAACCAACCTGCTGCAACGGCACTGAATACAACAATAACCTC1120.16999833037354098No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1100.1669626459025849No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC970.1472306968413703No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTCCCTTGATAAAG960.14571285460589228No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC960.14571285460589228No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA910.13812364342850203No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAAT900.136605801193024No Hit
CGATAGAACAAAAATTAGACAGCTGCCTAATCTTCTCCGAGGATACGAAC900.136605801193024No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT860.13053443225111183No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT840.1274987477801557No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA830.1259809055446777No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC820.12446306330919964No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT820.12446306330919964No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC800.12142737883824356No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT790.11990953660276552No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT780.11839169436728746No Hit
GAACAAAATAGTACCAAATTGAGTACTATTTCCTTTTATTCATCCATTAT780.11839169436728746No Hit
GGGATTTTGAGGATGAAGTAACCGCCCTTTGTATTCAGGGTCCATTAGAT710.10776679871894117No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT680.103213272012507No Hit
CCATCTGCTACTAATAGACCATCTCTTCCTTTAGTGTTCTCCCATAACTC670.10169542977702897No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA660.10017758754155093No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGC100.0071125063144.750181
GAGCAGA151.2332766E-4144.750181
CGGTGCA100.0071125063144.750182
CGTGCCT100.0071125063144.750183
ACGTGCC100.0071125063144.750182
CAGCCTT100.0071125063144.750185
GTAACAA750.0135.100194
TAACAAG850.0119.206035
TAGTAAC1200.096.500122
TAGAAAC406.1162973E-790.4688642
ACAAGAG1150.088.108817
GAAACAC451.2326691E-680.416774
GCAGAAG300.001940925772.375093
AGTAACA1650.070.181913
GTAGTAA1850.066.506841
AGAAGCG350.003576074762.0357935
GAAGCGG350.003576074762.0357936
AAACACG606.804703E-660.3125765
ACACGAG805.858801E-754.281327
CAGAAGC400.006067159654.281324
Produced by FastQC (version 0.11.2)