FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_468.352000000a1142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_468.352000000a1142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89668
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT6190.6903243074452424No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2240.24981041174108937No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2200.24534951153142703No Hit
TAATATATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACC2040.2275059106927778No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA1880.20966230985412856No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1780.1985100593299728No Hit
GGTGGATTAATACCTTTTTGCCAGGATATCATTGATTCATTAGACCGACC1780.1985100593299728No Hit
CAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTT1650.1840121336485703No Hit
GGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGAATTGCAACCAG1480.16505330775750546No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA1470.1639380827050899No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1230.13717268144711603No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1190.13271178123745372No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT1180.13159655618503815No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1140.12713565597537582No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG1130.12602043092296025No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT980.10929205513672659No Hit
GCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGAATTGCAACCAGC970.108176830084311No Hit
CCATCTGCTACTAATAGACCATCTCTTCCTTTAGTGTTTTCCCATAACTC950.10594637997947987No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC940.10483115492706428No Hit
ATATATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCC930.10371592987464871No Hit
GGGATTTTGAGGATGAAGTAACCGCCCTTTGTATTCAGGGTCCATTAGAT910.10148547976981755No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT900.10037025471740196No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCA100.007115629144.757954
CGGTGCA403.274181E-11126.66322
AAACACG550.0118.4383245
GTAACAA550.0118.4383244
CAGCCTT256.4862197E-6115.806365
TAGAAAC600.0108.568472
GAAACAC650.0100.217044
TAGTAAC800.090.473722
AACACGA750.086.854776
ACACGAG801.8189894E-1281.4263467
CACGAGC801.8189894E-1281.4263468
ACAAGAG801.8189894E-1281.4263467
GAGCATC451.2359396E-680.4210744
CAGAGCA451.2359396E-680.4210742
GCGGTGC1000.079.616871
TTCGGAT1200.078.41056145
AGCATCT651.5625119E-977.946595
TTATTCA1350.075.059689
ATTACAA300.001942812872.3789756
ACAGCCT406.685287E-572.3789754
Produced by FastQC (version 0.11.2)