FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_463.352000000a1135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_463.352000000a1135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31713
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT1160.3657805947087945No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT1000.31532809888689184No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT830.2617223220761202No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC630.19865670229874183No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTCCCTTGATAAAG440.1387443635102324No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC420.13243780153249457No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC420.13243780153249457No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT360.11351811559928106No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT360.11351811559928106No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT350.11036483461041213No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC340.1072115536215432No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATCG100.007095826144.74606145
GAGCAGA100.007095826144.746061
GCAGAAG100.007095826144.746063
CAGAAGC100.007095826144.746064
GAAACAC151.2274281E-4144.746054
TAACAAG450.0128.663165
GTAACAA450.0128.663164
TAGAAAC203.858048E-4108.559552
AAACACG203.858048E-4108.559555
AACACGA203.858048E-4108.559556
TAGTAAC553.6379788E-12105.269862
AGTAACA651.4551915E-1189.074493
AGGTTTG259.367525E-486.84764145
ACAAGAG755.2750693E-1177.19797
TTATTCA406.621495E-572.373039
ACACGAG300.001931824472.3730247
TAAGTGA300.001931824472.3730245
GCGTTTG300.001931824472.3730241
AAGTGAC300.001931824472.3730246
ATATTTA350.003559369162.034026
Produced by FastQC (version 0.11.2)