000000000-B56MK_l01n02_s1_460.352000000a1128.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-B56MK_l01n02_s1_460.352000000a1128.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	63168
Sequences flagged as poor quality	0
Sequence length	151
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA	153	0.24221124620060788	No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT	151	0.23904508611955422	No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA	138	0.21846504559270516	No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG	132	0.20896656534954405	No Hit
GTCTATTGGAATTGAAGGGTTTGAGCCATACTGTATGAACAGTTCCTCAA	124	0.19630192502532928	No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC	119	0.18838652482269502	No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT	112	0.1773049645390071	No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG	104	0.1646403242147923	No Hit
GTATATGCATACAATAGGATATTATTCATCCATAAAAGGGAATGAAGTAT	92	0.14564336372847012	No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC	92	0.14564336372847012	No Hit
ATATAAAGAGATCCATATCTAGACACATTTAAATCAAAGTGTTGAAATAC	90	0.14247720364741642	No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG	89	0.14089412360688958	No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC	86	0.136144883485309	No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT	83	0.13139564336372847	No Hit
CCCTAAGGAAGAGGAAGAATATGATTTTCAGACTTGTCATGTTATGTTAC	81	0.12822948328267478	No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA	80	0.12664640324214793	No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA	79	0.12506332320162108	No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG	79	0.12506332320162108	No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT	78	0.12348024316109422	No Hit
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA	77	0.12189716312056738	No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG	76	0.12031408308004053	No Hit
ATACAGTGTCTGAGGAAGCCCAGATATTGGACACACTAGACAAATACTTT	76	0.12031408308004053	No Hit
CTTTATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAG	76	0.12031408308004053	No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC	76	0.12031408308004053	No Hit
GAGCCATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAA	75	0.11873100303951367	No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA	74	0.11714792299898683	No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT	74	0.11714792299898683	No Hit
ACACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGA	74	0.11714792299898683	No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG	72	0.11398176291793312	No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG	71	0.11239868287740629	No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT	70	0.11081560283687944	No Hit
ATTTTAAATATGTTTGAAGAATTAAAGAAACCATGTCTTTAAATGTCTTA	70	0.11081560283687944	No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG	68	0.10764944275582573	No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC	68	0.10764944275582573	No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG	68	0.10764944275582573	No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA	67	0.10606636271529889	No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT	65	0.10290020263424518	No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT	64	0.10131712259371835	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAACAC	15	1.2330248E-4	144.7506	4
GGTCGTT	10	0.0071117613	144.75058	7
GTAACAA	105	0.0	89.60751	4
TAGTAAC	125	0.0	75.27031	2
GTACCAA	30	0.0019405336	72.3753	1
AAACACG	30	0.0019405336	72.3753	5
AGTAACA	130	0.0	72.3753	3
TAACAAG	150	0.0	67.55028	5
ACAAGAG	150	0.0	62.725258	7
GTATAGA	40	0.006065944	54.28147	6
AACACGA	40	0.006065944	54.28147	1
GTAGTAA	200	0.0	50.66271	1
ACACGAG	45	0.009663189	48.2502	2
CACGAGC	45	0.009663189	48.2502	3
CAAGAGC	135	1.0153599E-8	42.88906	8
CAAGAGG	85	5.33859E-5	42.573704	8
AACAAGA	240	0.0	42.21892	6
GTAGAAA	75	0.0014853891	38.60016	1
GATATAA	125	5.1516783E-4	28.950119	1
GACTCAT	100	0.0060829916	28.950119	8

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content