FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_451.352000000a111b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_451.352000000a111b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65073
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2260.3473022605381648No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1860.28583283389424186No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1740.26739200590106493No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1580.24280423524349576No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1420.21821646458592658No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1330.20438584359104392No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1260.19362869392835738No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1200.18440827993176895No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA1160.17826133726737664No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1140.1751878659351805No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1060.1628939806063959No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA980.15060009527761128No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC970.1490633596115132No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT970.1490633596115132No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC960.14752662394541513No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT950.14598988827931705No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC940.144453152613219No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA920.14137968128102285No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT910.13984294561492477No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT900.1383062099488267No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG850.13062253161833634No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG840.12908579595223826No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC820.1260123246200421No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG810.12447558895394403No Hit
AAATACAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGA780.1198653819556498No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT770.11832864628955173No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG750.1152551749573556No Hit
GATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG750.1152551749573556No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA720.11064496795906137No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG720.11064496795906137No Hit
GGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATG720.11064496795906137No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC710.10910823229296329No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT690.10603476096076714No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG690.10603476096076714No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC680.10449802529466906No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG670.102961289628571No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGA100.0071119694144.75251
CACGCAC202.133571E-6144.75253
GCACGCA253.709647E-8144.75252
CCAGCGG100.0071119694144.75255
CGCACTT202.133571E-6144.75255
ATCGCTT100.0071119694144.75258
ACACGAG351.897206E-9124.073567
AAACACG351.897206E-9124.073565
AGCACGC301.1012162E-7120.6270751
AACACGA404.800313E-9108.564386
ACGAGCC352.7605347E-7103.394649
CACGAGC451.0884833E-896.501678
GTAGAAA702.910383E-1182.715711
GAAACAC554.3815817E-878.955914
GTGTAAT300.001940696272.376241
ACGCACT451.1955825E-464.334444
CAGAAGC400.006066445754.282193
AGCATCT805.857037E-754.282195
GTAACAA701.695422E-551.697324
TAGTAAC701.695422E-551.697322
Produced by FastQC (version 0.11.2)