FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_450.352000000a110e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_450.352000000a110e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62309
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT3340.5360381325330209No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2550.4092506700476657No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2530.4060408608708212No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA2230.3578937232181547No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC2040.3274005360381326No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1790.2872779213275771No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1760.2824632075623104No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1710.2744386846201993No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1680.26962397085493267No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1630.2615994479128216No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA1620.25999454332439936No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG1500.24073568826333275No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1490.23913078367491053No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC1480.2375258790864883No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA1440.23110626073279944No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG1420.227896451555955No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT1350.21666211943699945No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT1300.2086375964948884No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG1270.20382288272962173No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT1260.20221797814119952No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG1250.2006130735527773No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC1230.19740326437593286No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC1210.19419345519908843No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1210.19419345519908843No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA1190.190983646022244No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1110.17814440931486622No Hit
CTGCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAA1050.1685149817843329No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC1050.1685149817843329No Hit
ACACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGA1050.1685149817843329No Hit
AAATACAATTGGACCGATTACCCCTCAACACCAGAGAGGTGCCTTGATGA990.15888555425379963No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG990.15888555425379963No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG980.15728064966537741No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT970.15567574507695517No Hit
AATAGCAACTGTACGAAATACAATTGGACCGATTACCCCTCAACACCAGA960.15407084048853295No Hit
CTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATGATAATGAT930.1492561267232663No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT910.14604631754642186No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGTTTGACA900.14444141295799964No Hit
CAATTGGACCGATTACCCCTCAACACCAGAGAGGTGCCTTGATGACATAG900.14444141295799964No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT890.14283650836957743No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT890.14283650836957743No Hit
AGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGA890.14283650836957743No Hit
GGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCAGAG870.139626699192733No Hit
TTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCA870.139626699192733No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAA860.13802179460431077No Hit
GTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACCAGTC850.13641689001588855No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGT840.13481198542746634No Hit
GAGCCATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAA830.13320708083904412No Hit
CCTATTTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTA830.13320708083904412No Hit
ACATAGAGGAAGAACCAGAGGATGTTGATGGCCCAACTGAAATAGTATTA830.13320708083904412No Hit
GTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAACAACATGACCACAA800.12839236707377746No Hit
GATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG800.12839236707377746No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC790.12678746248535525No Hit
GATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTTACCAA790.12678746248535525No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC770.1235776533085108No Hit
GTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTTAC770.1235776533085108No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT770.1235776533085108No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT750.12036784413166637No Hit
ACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAG750.12036784413166637No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG750.12036784413166637No Hit
CATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTCA740.11876293954324416No Hit
ATAAAAAGGGATATGCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGG740.11876293954324416No Hit
CTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCA730.11715803495482194No Hit
TGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGA730.11715803495482194No Hit
CCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGATGG720.11555313036639972No Hit
GGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGTCAAGAATAAA720.11555313036639972No Hit
GGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAACAACATGA720.11555313036639972No Hit
ATTTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTATGA720.11555313036639972No Hit
ATTCTGGAGTGCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCC720.11555313036639972No Hit
GTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGGTC710.1139482257779775No Hit
ATTTCATACAATGTTTCTAATAATGATAATGATACAACCATATCAGCTAT700.11234332118955528No Hit
GAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTT690.11073841660113307No Hit
ACTCTGCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAA680.10913351201271085No Hit
GCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCCCGGTCAAGAC670.10752860742428863No Hit
ACTGAAATAGTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGAT650.1043187982474442No Hit
GCTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATC640.10271389365902198No Hit
CACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGAT640.10271389365902198No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC630.10110898907059976No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT630.10110898907059976No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC630.10110898907059976No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG630.10110898907059976No Hit
GATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCC630.10110898907059976No Hit
ACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAGTGCTATGAAAG630.10110898907059976No Hit
TTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTATGAAC630.10110898907059976No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGA100.007111207144.75284
GTTCTAC100.007111207144.75281
CGAGCCA100.007111207144.75285
TCGAGCC100.007111207144.75284
CTACTCT100.007111207144.75289
GAGCAGA301.1006523E-7120.6273351
CGGTGCA256.471697E-6115.8022462
GGTAGTA203.8750898E-4108.5646062
AACACGA203.8750898E-4108.5646066
GTAACAA950.091.422834
GAAACAC259.4087457E-486.8516854
AAACACG259.4087457E-486.8516855
CACGAGC259.4087457E-486.8516858
GAGCATC259.4087457E-486.8516854
CCGGGGT259.4087457E-486.851685145
TAGAAAC353.4409837E-582.71592
TAACAAG1050.082.715895
TAGTAAC1200.078.407772
AGTAACA1350.075.057013
ACACGAG300.001940292472.37647
Produced by FastQC (version 0.11.2)