FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_445.352000000a10f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_445.352000000a10f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182099
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA6290.34541650420924885No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC3780.2075793936265438No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT2940.16145063948731186No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2710.14882014728252213No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG2670.14662353994255872No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC2560.1405828697576593No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT2420.1328947440677873No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2110.11587103718307076No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT2060.11312527800811648No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA2030.11147782250314389No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT1940.10653545598822618No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTTATTGAATTAATGGAT1890.1037896968132719No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1860.10214224130829933No Hit
CCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATAT1850.10159308947330847No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1830.10049478580332677No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT1830.10049478580332677No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCATC151.2370986E-4144.742394
AAGCGGA151.2370986E-4144.742392
AGCGGAG151.2370986E-4144.742393
AGGATTG800.0144.742395
GTAACAA2000.0119.412454
GAGCAGA203.8883326E-4108.5567861
CGGTGCA501.9645086E-10101.319662
GAAACAC950.099.0342564
GCGGAGC259.440788E-486.845424
GGATTGA1350.085.773266
ACAAGAG2800.085.294627
TAACAAG3050.078.303255
AGCATCT1050.068.924945
AAACACG1450.064.8845145
AACACGA1450.064.8845146
CGGTGCG350.003587000862.0324487
GGTGCGT350.003587000862.0324488
TAGAAAC1550.060.6984142
ACGAGCC606.8552326E-660.3093269
GAGCATC851.315675E-859.5998084
Produced by FastQC (version 0.11.2)