FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_443.352000000a10e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_443.352000000a10e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134033
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2580.1924899092014653No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2250.167869106861743No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2160.1611543425872733No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1840.13727962516693648No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1760.1313109458118523No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA1740.12981877597308125No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1600.11937358710168391No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1470.10967448314967211No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1470.10967448314967211No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1440.10743622839151552No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAG100.00711966144.757083
CGATGAT100.00711966144.757085
AGCACGC100.00711966144.757081
CGGTGCA403.274181E-11126.6624452
CGCACTT203.8844973E-4108.567815
GTAACAA2400.093.488964
TAACAAG2550.087.98965
TAGTAAC2600.083.51372
CGAGCAT353.455015E-582.718345
AGTAACA3000.074.791173
CTATGTG554.0828836E-665.7986761
ACAAGAG3700.062.597667
GGTGCGT350.003583491562.0387546
GGTGCAT702.3462235E-762.0387543
TAGATGT350.003583491562.0387548
ACACGAG606.841261E-660.3154562
AACACGA606.841261E-660.3154561
GAGCAGA851.3111276E-859.605861
CGGTGCG400.006079694754.2839055
AAACACG553.2356085E-452.638945
Produced by FastQC (version 0.11.2)