FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_436.352000000a10d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_436.352000000a10d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137285
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2800.20395527552172485No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2700.19667115853880612No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2250.16389263211567176No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC2030.14786757475325055No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT1990.14495392796008305No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1970.1434971045634993No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG1700.12382998870961869No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1680.12237316531303494No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1590.11581746002840805No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1530.11144698983865681No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT1500.10926175474378118No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1490.10853334304548931No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC1470.10707651964890558No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA1430.10416287285573807No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1430.10416287285573807No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTCG100.0071201245144.75519145
CGGTGCA352.7767783E-7103.396572
TAGTAAC2150.094.259192
GTAACAA2200.088.827044
TCGTCCA259.4324804E-486.853124
GTCGTCC259.4324804E-486.853123
CTGTCGT300.00194516972.3775941
TAACAAG2700.072.3775945
ACAAGAG2850.071.107827
GGTGCAT451.2001993E-464.335653
CGATAGA702.3468601E-762.037941
AGTAACA3400.059.605083
AACACGA805.904749E-754.2831956
ACGTGCA450.00968600448.251732
CGTCCAG450.00968600448.251735
ATATTGA450.00968600448.251731
AACAAGA4200.048.2517286
AAACACG752.5644891E-548.2517285
TAAATAC1056.9398084E-848.2517284
CAAGAGC3450.046.1538288
Produced by FastQC (version 0.11.2)