FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_434.352000000a10c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_434.352000000a10c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101768
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2530.24860466944422607No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT2360.23190000786101722No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA1840.18080339595943715No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1540.15132458140083327No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1380.13560254696957785No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1340.13167203836176403No Hit
GGATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAGGCCAAATG1250.12282839399418286No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1120.11005424101878784No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT1060.10415847810706705No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1050.10317585095511358No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1020.10022796949925321No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGC151.2350515E-4144.755781
GAGCAGA256.4909545E-6115.804621
GTAACAA1250.098.433934
TAGTAAC1200.090.472362
GGAGCAT259.425267E-486.853463
AAACACG502.2746462E-886.853465
GCGGAGC259.425267E-486.853461
CGGTGCA353.451163E-582.717582
GAGCATC703.092282E-1182.717584
GAAACAC554.405956E-878.9576954
AGCATCT755.638867E-1177.203085
TAGAAAC608.052666E-872.377892
CGGAGCA300.001943685972.377892
AACACGA608.052666E-872.377896
GTGCGTT406.690126E-572.3778841
AGTAACA1750.070.3099443
TAACAAG1900.064.759165
GGTGCAT451.1986882E-464.33593
ACGTGCA350.00358114162.038192
ACAAGAG2050.060.0206837
Produced by FastQC (version 0.11.2)