FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_428.352000000a10bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_428.352000000a10bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60234
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC690.11455324235481622No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA100.007110174144.755811
TAGTAAC950.0114.280912
GTCTCGC203.874127E-4108.5668641
GTAGTAA1150.094.405971
GTAACAA1350.091.142554
CGCACTT608.221832E-1084.4408955
ACAGCTA554.3752152E-878.957714
GCACGCA702.7903297E-972.377912
TCGCTCT300.001939812972.377914
TCTCGCT300.001939812972.377912
CTCGCTC300.001939812972.377913
CGGTGCA300.001939812972.377912
ACGCACT754.816684E-967.552714
GGTCTGC350.003574031662.038208145
TAACAAG2000.061.5212255
AGTAACA1900.060.9498183
ACAAGAG2100.055.1450737
TTGCTCT400.006063705354.2834324
CGCTCTG400.006063705354.2834325
CACGCAC953.1146556E-853.331093
Produced by FastQC (version 0.11.2)