FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_425.352000000a10a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_425.352000000a10a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93638
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2070.22106409790896858No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1670.17834639782994083No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1580.1687349153121596No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1410.1505798927785728No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1400.1495119502765971No Hit
ATATTGTAGTTGGGTCTCTACAGTGAGGCAGGATCTGCCCAGGCAGAGGG1380.14737606527264574No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1380.14737606527264574No Hit
GTAGTAACAAGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC1350.14417223776671864No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1260.13456075524893737No Hit
CCTAAGGGGTAGCCACTACTCAGCACCCACTTTTTGTTGCCAGACCTTCC1230.1313569277430103No Hit
CATTTAGGTTTCTGGTCTCTGATAATTTGGAAGGTCTGGCAACAAAAAGT1190.12708515773510756No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1160.12388133022918045No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT1140.12174544522522907No Hit
CCCCTTAGGTGGACCCTAAACTCTTCACTATAACTTCCCTGCCCAGCACC1140.12174544522522907No Hit
GTAGAAACACGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC1120.1196095602212777No Hit
CTACAGTGAGGCAGGATCTGCCCAGGCAGAGGGAAGAGCAGGCAGAGCCG1090.11640573271535061No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1060.11320190520942354No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG990.10572630769559367No Hit
CAGTAAAGTCTGAGCTAAAATCGGAGGCTCTCGAGCCACATCCACCTCGC990.10572630769559367No Hit
TCACAGAGCACCCCCTAGTCTTGGAAACCCTTCCTCAAAGAGTCACCTGG960.1025224801896666No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA950.1014545376876909No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCG608.2945917E-1084.4350055
TACTGCA300.00194373372.372862
TATTAGT608.049574E-872.372861
GTAACAA1450.064.886024
AGCATCT451.19859935E-464.3314365
GAGCATC451.19859935E-464.3314364
ACTGCAT350.003581228262.0338823
TAGTAAC1600.058.802952
ATTAGTA753.7696918E-757.898291
AAGAGCG502.0182352E-457.898299
CATAGAA400.006075873554.279648145
AGTAACA1850.050.85663
TAATAGC450.00967894948.2485774
GGTGTGC450.00967894948.2485778
GTATTAC450.00967894948.2485771
TGCCAGG450.00967894948.2485776
GCTGACC450.00967894948.248577145
TAACAAG2000.047.042365
ACACGAG1109.9667886E-846.0554583
AAACACG951.9252875E-645.7091751
Produced by FastQC (version 0.11.2)