FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_421.352000000a1093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_421.352000000a1093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57791
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAACCTGTGTTTGAGAAAGGCTAGCTCTGTTTCTATTGCCATAATT950.16438545794327836No Hit
ACAACAGCTTGTGCAAAGGCCCTGAGGCATGAATGATTAAAAGATATAAT870.15054247201121282No Hit
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT750.1297779931131145No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC720.12458687338858991No Hit
ACAACAGCCAACATAGATACCACAGGGCACAAAAGGCCAGGAATACAAAA700.12112612690557353No Hit
GTGCTACTCCTGTGACACGCAATGTCTTCTAGCTTGTTGGTTCCACAGTT620.10728314097350798No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCTG203.8776494E-4108.536365
GCACGCA553.6379788E-12105.2473752
CGCACTT553.6379788E-12105.2473755
GTAGTAA850.0102.151871
TAGTAAC900.096.476772
TCTGTCG259.414962E-486.829098
TCGCTCT259.414962E-486.829094
TCTCGCT259.414962E-486.829092
CTCGCTC259.414962E-486.829093
GTAACAA1100.085.513494
TAACAAG1250.075.251885
CTGTCGT300.001941574572.3575741
ACAGCTA300.001941574572.3575744
AGCTAAC300.001941574572.3575746
GCACTTG406.674919E-572.3575746
ACGCACT851.6734703E-1068.101254
CACGCAC851.6734703E-1068.101253
AGCACGC754.822141E-967.533741
TTAGGCT451.1959748E-464.31784145
GTAGAAA808.032657E-963.3128781