FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_420.352000000a1086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_420.352000000a1086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118780
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT1770.14901498568782623No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1580.13301902677218386No Hit
GCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT1520.12796767132513892No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1510.1271257787506314No Hit
ATATTTGCTTTCTCCCCTGGTTTTAGCTTTTCAAGTTCTTCAATGGTTTT1490.12544199360161645No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT1450.12207442330358646No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG1440.12123253072907896No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC1400.117864960431049No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA1320.11112981983498904No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGA100.007119766144.749472
CCGCTCG100.007119766144.74947145
TTACGAA100.007119766144.749473
CGCACTT100.007119766144.749475
AGCACGC100.007119766144.749471
CGGTGCA301.10703695E-7120.624572
AAACACG800.090.468425
CGACGAC259.4308064E-486.84968145
GAAACAC900.080.4163744
CAGCCTT406.6961766E-572.374735
ACACGAG955.456968E-1268.565547
AACACGA955.456968E-1268.565546
GGTGCAT554.0812956E-665.795223
ACGAGCC451.1997698E-464.33314
GCGGTGC808.114512E-963.3278961
CACCTAG350.003583233862.035491
GTAGTAA2150.060.59281
TAGAAAC1500.057.8997882
GTAACAA2450.056.1273464
CACGAGC1206.002665E-1154.2810558
Produced by FastQC (version 0.11.2)