FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_418.352000000a1079.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_418.352000000a1079.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304064
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA7780.25586718585560936No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT7040.2315302041675437No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG6980.22955693538202482No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG5250.17266101873289835No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC5250.17266101873289835No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC4970.16345243106714377No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA4970.16345243106714377No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT4480.14733740265207323No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC4280.1407598400336771No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA4130.13582666806988003No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG4020.13220900862976215No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG4010.13188013049884234No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT3890.12793359292780468No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC3670.12069827404756893No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT3650.12004051778572933No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA3530.11609398021469165No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT3480.1144495895600926No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG3450.11346295516733318No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT3420.11247632077457377No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT3300.1085297832035361No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC3290.10820090507261629No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG3280.1078720269416965No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA3260.10721427067985688No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC3250.10688539254893707No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC3220.10589875815617765No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG3160.1039254893706588No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG3100.10195222058513996No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT501.8189894E-12115.779915
AACACGT203.8927404E-4108.5436556
CGGTGCA553.6379788E-12105.254462
AGCACGC553.6379788E-12105.254461
ACGTGCA406.176615E-790.453042
GCACGCA703.092282E-1182.699932
GTAACAA4400.082.230054
ACGCACT755.638867E-1177.18664
TAGTAAC4850.074.600452
CGGAAAA608.119059E-872.3624341
AAACGCT608.119059E-872.3624347
AACGCTC608.119059E-872.3624348
AGCATCT1150.069.216255
GAGCATC1050.068.916614
ACGAGCC651.4132456E-766.79613
AGTAACA5700.063.4758223
CGAGCAT808.183633E-963.317133
AAACACG1051.6370905E-1162.024955
TAACAAG5950.060.8087735
ACAAGAG6450.056.0949177
Produced by FastQC (version 0.11.2)