FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_412.352000000a106c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_412.352000000a106c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157015
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG4550.28978123109257076No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG2950.1878801388402382No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT2470.1573098111645384No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT2400.15285163837849888No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2330.1483934655924593No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA2250.1432984109798427No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG1910.12164442887622202No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG1910.12164442887622202No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1880.1197337833964908No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG1840.11718625609018246No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT1710.10890679234468045No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC1640.10444861955864089No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1610.10253797407890966No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC1600.10190109225233258No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1580.10062732859917843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTT100.0071227937144.743647
ACGTGCA151.2367341E-4144.743642
CGGTGCA352.7799797E-7103.388312
AAACACG451.0990334E-896.495755
AACACGA451.0990334E-896.495756
TATAGAG451.2410255E-680.4131245
ACACGAG554.424146E-878.951077
CACGAGC554.424146E-878.951078
GAAACAC554.424146E-878.951074
GAGCATC1850.074.3278054
GCGGTGC502.3214616E-672.371821
ACGCACT300.001946309172.371824
GGTGCAT502.3214616E-672.371823
GCGGAGC406.7042376E-572.371821
CACGTGC300.001946309172.371821
CAGAGCA1950.066.8047562
GCAGAGC2050.063.5459821
AGCACGC350.003585957562.032991
GTAACAA2000.061.516044
AGCATCT2600.058.454165
Produced by FastQC (version 0.11.2)