FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_403.352000000a1036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_403.352000000a1036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91487
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC2270.24812268409719415No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1400.15302720605113296No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1360.14865500016395772No Hit
GATAAAGTTCTTCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAA1350.1475619486921639No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1230.13444533103063824No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1190.130073125143463No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1080.11804955895373112No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1000.10930514717938068No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT1000.10930514717938068No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT980.10711904423579305No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA980.10711904423579305No Hit
GAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTT950.10383988982041165No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG920.10056073540503023No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT920.10056073540503023No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC100.0071185357144.739765
GCGGAGC403.274181E-11126.6472851
TAGAAAC451.0941221E-896.4931642
CGGAGCA301.6059246E-596.493162
GTAACAA1050.089.60084
ACACGAG451.2370638E-680.410977
TTTAAGA406.69017E-572.369889
CTTTAAG406.69017E-572.369888
ACGAGCA406.69017E-572.369889
AACACGA406.69017E-572.369886
TAACAAG1500.067.545225
TAGTAAC1450.064.883342
AAACACG451.1986979E-464.328775
TAGACTA400.006076420754.277414
CACGAGC553.2320753E-452.6326338
GAAACAC553.2320753E-452.6326334
ACAAGAG1800.052.267137
AGTAACA1850.050.8545043
GGGAACC450.00967982548.2465828
GCAGAGC604.965184E-448.246581