FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_402.352000000a1029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_402.352000000a1029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137254
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2680.19525842598394216No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1990.14498666705524066No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1940.14134378597345068No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1900.1384294811080187No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1860.13551517624258674No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1850.13478660002622875No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1620.11802934704999489No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1550.11292931353548893No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1450.10564355137190902No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1440.10491497515555102No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1430.10418639893919303No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1430.10418639893919303No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA1430.10418639893919303No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC1390.10127209407376106No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGATT203.885719E-4108.560655
CACAGGA203.885719E-4108.560653
GCGGAGC203.885719E-4108.560651
CGGAGCA203.885719E-4108.560652
GAGCAGA301.6083804E-596.498361
GAAGCGG259.434463E-486.848526
AGAAGCG300.001945576672.373775
CAGCATC350.003584613362.034664
GTAACAA1200.060.3114784
CCATATG502.0214483E-457.8990143
ATGGTGC553.236939E-452.635478
GATTACA553.236939E-452.635472
CACGAGC450.00968802648.2491843
TAACAAG1551.4551915E-1146.6927535
CAGAAGC803.756189E-545.233614
ATTGTAC855.3722986E-542.5728073
TAGTAAC1903.6379788E-1241.90062
ATATGAT1053.84578E-641.3564383
CACTGCC907.525597E-540.2076537
GCACAGC951.0348262E-438.0914571
Produced by FastQC (version 0.11.2)