FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_398.352000000a101c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_398.352000000a101c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5782
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTTAAGATTAGGA180.3113109650639917No Hit
AGCTTATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTT180.3113109650639917No Hit
GAGTAACACCTGGGAAAGCTTCACCAGAGGGTCCTTTTCCACCACAGCAA150.2594258042199931No Hit
GAATAAGCAGGAGTATTGCTGTGGTGGAAAAGGACCCTCTGGTGAAGCTT150.2594258042199931No Hit
TTCTTTCACCCTCCAGAAAGTCAAAAAGCAAAATGCCTTGAGCATCCCAA140.24213075060532688No Hit
GTCAAGAGCAAAGATCATGGCAACATTTTGGGATGCTCAAGGCATTTTGC130.2248356969906607No Hit
ACCTAATTCATCCCTAAAAAACTAAGGGTCCTGGGAATTTAACCATGTCA130.2248356969906607No Hit
AATCAGGACTGAAAGGTGGATGCCCAATGATTTCCCATCAAAACTCTTGA130.2248356969906607No Hit
GCATTGACATGGTTAAATTCCCAGGACCCTTAGTTTTTTAGGGATGAATT120.20754064337599448No Hit
GCTTATTCTCATCAAATGTAGGCAACTTTTCAAGAGTTTTGATGGGAAAT110.19024558976132827No Hit
AATTAAAGTTGTGCAAACTTTCCACTTGATGAGTGCCGAAACCATTGTGT110.19024558976132827No Hit
CCCTAACACATAGGTAATTAGATTAAACATAAATGGTTCTGAAATAACAA110.19024558976132827No Hit
TCTTTAGGGTTGTGCTTTCAAAGGAAGAAGGATACTCTTGTCTTTCTCCT110.19024558976132827No Hit
CTTTTTTACATTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTT110.19024558976132827No Hit
TCTGAAGACAAAGCACAATCAAAGCAATGGCTACCAAGAGGTGGAAATAG100.17295053614666206No Hit
AGTGAGGAGAGAGGGAAAACTTGTGGAAAAACTGAATTACATAATGAACA100.17295053614666206No Hit
CTTTTATAAGTGGTACTACCAGCCACCTAATTCATCCCTAAAAAACTAAG100.17295053614666206No Hit
GAAGAAAAATGGGGCCCTTTACAGATTTTTTAAGATTAGGAAACAAGAAG100.17295053614666206No Hit
ATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTTGATGA100.17295053614666206No Hit
CATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTAACA100.17295053614666206No Hit
TGCTTATTCTCATCAAATGTAGGCAACTTTTCAAGAGTTTTGATGGGAAA100.17295053614666206No Hit
GTTAAATTCCCAGGACCCTTAGTTTTTTAGGGATGAATTAGGTGGCTGGT100.17295053614666206No Hit
TCTGGAGGGTGAAAGAATGATAACATCTGCTCATTATGAGTGCGTTTTGA100.17295053614666206No Hit
CATCTGCTCATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCA100.17295053614666206No Hit
GAAAGGTGGATGCCCAATGATTTCCCATCAAAACTCTTGAAAAGTTGCCT90.15565548253199585No Hit
GGCTAACCTTCTCAAAACGCACTCATAATGAGCAGATGTTATCATTCTTT90.15565548253199585No Hit
GGTAAAGCCATGTCCTATTGCAGTTCTTCCAAGAAGTGCTTCAGGATCTT90.15565548253199585No Hit
CTTAAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCA90.15565548253199585No Hit
GTTTACAGCAGTGAGGAGAGAGGGAAAACTTGTGGAAAAACTGAATTACA90.15565548253199585No Hit
CTTTTGGTCCGTGCGCTGCCGTAGAACGGTAGGTACGTCCTTGAAGATCA80.13836042891732964No Hit
CTGGTGAAGCTTTCCCAGGTGTTACTCTGCTCATGCTCTGGCTAACCTTC80.13836042891732964No Hit
GCCTTGAGCATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCTG80.13836042891732964No Hit
GATGTTATCATTCTTTCACCCTCCAGAAAGTCAAAAAGCAAAATGCCTTG80.13836042891732964No Hit
GCTTTCAATGGAAATTTTAAACAAGGGGGATTAAGATCCTGAAGCACTTC80.13836042891732964No Hit
GAATTAGGTGGCTGGTAGTACCACTTATAAAAGTGTCTTGAACTTGATGG80.13836042891732964No Hit
TGTCAATGCAATCTTTTTTACATTATTAACTGAAGAAAAATGGGGCCCTT80.13836042891732964No Hit
GGCTTTACCAGTATGATTCTGAAGACAAAGCACAATCAAAGCAATGGCTA80.13836042891732964No Hit
AAACAACAGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCT80.13836042891732964No Hit
GAATTTAACCATGTCAATGCAATCTTTTTTACATTATTAACTGAAGAAAA80.13836042891732964No Hit
ACATAATGAACATAAAAAATGTGAACTTTATTTTTTTAACTTAAGTTCCA80.13836042891732964No Hit
GTGGTACTACCAGCCACCTAATTCATCCCTAAAAAACTAAGGGTCCTGGG70.12106537530266344No Hit
GGTAATTAGATTAAACATAAATGGTTCTGAAATAACAAAATTTTATTTAA70.12106537530266344No Hit
GTGTTACTCTGCTCATGCTCTGGCTAACCTTCTCAAAACGCACTCATAAT70.12106537530266344No Hit
AATGTAGGCAACTTTTCAAGAGTTTTGATGGGAAATCATTGGGCATCCAC70.12106537530266344No Hit
GGGTGAAAGAATGATAACATCTGCTCATTATGAGTGCGTTTTGAGAAGGT70.12106537530266344No Hit
CTTTAAATGTCTTACAAAATAGAAACTTTCAATAAAGAGATAGAAACTGC70.12106537530266344No Hit
GCTTATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTTG70.12106537530266344No Hit
GCTCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAAC70.12106537530266344No Hit
ACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAAAAATTAA70.12106537530266344No Hit
ATCTTTGCTCTTGACTGGTCTGCATTTGCTTTGACTGGGCTATTTCCACC70.12106537530266344No Hit
CTTGATGAGTGCCGAAACCATTGTGTCCAGCTCAGCGGCAGACAAGAGCA60.10377032168799724No Hit
CAATAGGACATGGCTTTACCAGTATGATTCTGAAGACAAAGCACAATCAA60.10377032168799724No Hit
CTGCTAGGATGTCCCCCTTGTCCATGTTATCCAGGATGGCTCACCACCAT60.10377032168799724No Hit
GTATGATTCTGAAGACAAAGCACAATCAAAGCAATGGCTACCAAGAGGTG60.10377032168799724No Hit
ATGATTTCCCATCAAAACTCTTGAAAAGTTGCCTACATTTGATGAGAATA60.10377032168799724No Hit
GTAAAGGGCCCCATTTTTCTTCAGTTAATAATGTAAAAAAGATTGCATTG60.10377032168799724No Hit
CCTTTACAGATTTTTTAAGATTAGGAAACAAGAAGTCAGAAGGAGACAAA60.10377032168799724No Hit
GGTTAGCCAGAGCATGAGCAGAGTAACACCTGGGAAAGCTTCACCAGAGG60.10377032168799724No Hit
TTTATAAGTGGTACTACCAGCCACCTAATTCATCCCTAAAAAACTAAGGG60.10377032168799724No Hit
ATCAAAGCAATGGCTACCAAGAGGTGGAAATAGCCCAGTCAAAGCAAATG60.10377032168799724No Hit
GCTCTTGACTGGTCTGCATTTGCTTTGACTGGGCTATTTCCACCTCTTGG60.10377032168799724No Hit
GAGCAGATGTTATCATTCTTTCACCCTCCAGAAAGTCAAAAAGCAAAATG60.10377032168799724No Hit
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA60.10377032168799724No Hit
CCTAATTCATCCCTAAAAAACTAAGGGTCCTGGGAATTTAACCATGTCAA60.10377032168799724No Hit
TCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCTGCATTTGCTTT60.10377032168799724No Hit
ATATTAAACTCTTTGAAAACTCCATGCAGTCTTGGCTAAGGGAAGCCCAG60.10377032168799724No Hit
ATATTTAAGGGGGCAGCAGCACCTGGGTGCCAGCAGGAAGTCACGTGAAA60.10377032168799724No Hit
GTTCTGAAATAACAAAATTTTATTTAAAAATGGGAAAGATTAGTTTACAG60.10377032168799724No Hit
ACTTAAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCC60.10377032168799724No Hit
CTCCAGAAAGTCAAAAAGCAAAATGCCTTGAGCATCCCAAAATGTTGCCA60.10377032168799724No Hit
CTCATAATGAGCAGATGTTATCATTCTTTCACCCTCCAGAAAGTCAAAAA60.10377032168799724No Hit
GCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAAA60.10377032168799724No Hit
CTCTACATCTATCTATATTAAACTCTTTGAAAACTCCATGCAGTCTTGGC60.10377032168799724No Hit
GGCTACCAAGAGGTGGAAATAGCCCAGTCAAAGCAAATGCAGACCAGTCA60.10377032168799724No Hit
GAAAAGGACCCTCTGGTGAAGCTTTCCCAGGTGTTACTCTGCTCATGCTC60.10377032168799724No Hit
GCTCATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTA60.10377032168799724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAGAA100.006944649144.756524
AAAGAAG100.006944649144.756525
Produced by FastQC (version 0.11.2)