FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_395.352000000a100f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_395.352000000a100f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156888
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT2790.1778338687471317No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2480.1580745499974504No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA2170.13831523124776912No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2000.1274794758043955No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1990.1268420784253735No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1950.1242924889092856No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1860.11855591249808782No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1800.11473152822395595No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1650.10517056753862628No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1640.1045331701596043No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1640.1045331701596043No Hit
GGAACAGGCTACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAA1620.10325837540156035No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTA100.0071218396144.750084
CGGAGCA100.0071218396144.750082
CGGTGCA501.9645086E-10101.325052
GTAACAA2400.096.500054
AAACACG850.085.14715
GGTGCAT608.3491614E-1084.4375463
GAAGCGG353.457688E-582.714331
TAGAAAC900.080.416712
GAAACAC950.076.184254
TAGTAAC3150.073.523852
TAACAAG3250.073.4884955
AACACGA851.70985E-1068.117686
CATAACC1200.066.343796
ACAAGAG3600.066.343797
AACCTCC1350.058.972269
ACACGAG1007.239578E-1057.9000327
GTAGAAA1251.8189894E-1257.9000321
GGCGATA902.062552E-856.29171
CCTATAT400.00608278654.281284
GCGGTGC1353.6379788E-1253.6111451
Produced by FastQC (version 0.11.2)