FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n02_s1_390.352000000a0fe7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n02_s1_390.352000000a0fe7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129523
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC2780.2146336944017665No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1980.1528686024875891No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGCTCTTCCGTGACC1930.149008284242953No Hit
GATAAAGCTCTTCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAA1850.14283177505153524No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA1770.1366552658601175No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1690.13047875666869976No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1590.12275812017942758No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1590.12275812017942758No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1500.11580954733908264No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1490.11503748369015541No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC1400.10808891084981047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA351.9135769E-9124.0656362
GGAGCAT301.1078919E-7120.619383
AACGTAT203.8856774E-4108.557436
ACGTATT203.8856774E-4108.557437
CGGAGCA352.7772512E-7103.388032
AAACACG451.0975782E-896.495495
GAGCATC451.0975782E-896.495494
GAAACAC502.2808308E-886.845954
TCCAAAC259.434362E-486.845952
GCGGAGC451.2398104E-680.412911
GTAACAA1200.078.4025964
AGCATCT554.084426E-665.792385
AACACGA554.084426E-665.792386
CGTATTG350.003584578262.0328188
CACGAGC606.8438458E-660.309698
ACACGAG651.099793E-555.670487
GCCTGCC651.099793E-555.67048145
TAACAAG1700.055.3430065
TAGTAAC1850.054.767722
AGAAGCG400.006081535554.2787132
Produced by FastQC (version 0.11.2)